Journal: Nature protocols

Article Title: Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

doi: 10.1038/s41596-020-00441-w

Figure Lengend Snippet: Troubleshooting table

Article Snippet: Cell culture incubator (37 °C, 5% CO 2 ) Refrigerated centrifuges for 15- and 1.5-ml tubes and multiwell plates Biosafety cabinet Mechanical pipettes Pipette controller Sterile filter pipette tips Sterile wide-bore pipette tips (e.g., USA Scientific, 1011–8410) Sterile 50-ml centrifuge tube (e.g., Corning Inc., 352098) Sterile 15-ml centrifuge tube (e.g., Corning Inc., 352095) Sterile 1.5-ml microcentrifuge tubes (e.g., Eppendorf, 0030120086) 96-well Round Bottom Ultra-Low Attachment Microplate (Corning Inc., 7007) Transwell (Corning Inc., 3470) 6-well multiwell cell culture plate (e.g., Corning Inc., 353046) 24-well multiwell cell culture plate (e.g., Corning Inc., 353047) 24-well multiwell cell culture plates (VWR International, 10062–896) 12-well multiwell cell culture plates (VWR International, 10062–894) 96-well multiwell cell culture plate (e.g., Corning Inc., 353072) Serological pipettes Inverted microscope (e.g., Olympus, model CKX53) Stereomicroscope (e.g., Leica, S8APO) Cell counter Cell counter slides Pasteur pipette Micro knife (Fine Science Tools, 10315–12) Spot Plate (Fisher Scientific, 13–748B) Hydrophobic pen (ImmeEdge pen; Vector Laboratories, cat. no. H-4000) Disposable base molds (15 × 15 × 5 mm; Fisherbrand, cat. no. 22–363-553) Nikon A1 single-photon confocal microscope with NIS-Elements Advanced research imaging software Flow cytometer (BD Biosciences, BD FACS AriaII)

Techniques: Marker, Expressing, Recombinant, Concentration Assay, Cell Culture